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Literature summary extracted from

  • Nam, S.W.; Seo, D.W.; Sung, D.S.; Han, J.W.; Hong, S.Y.; Lee, H.W.
    Nitric oxide synthase from bovine pancreas: purification and characterization (1998), Arch. Pharm. Res., 21, 128-134.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 additional information Ngamma,Ngamma'-dimethyl-L-arginine has no inhibitory effect Bos taurus
1.14.13.39 Ngamma,Ngamma-dimethyl-L-arginine
-
 Bos taurus
1.14.13.39 Ngamma-monomethyl-L-arginine
-
 Bos taurus
1.14.13.39 Ngamma-nitro-L-arginine
-
 Bos taurus
1.14.13.39 Ngamma-nitro-L-arginine methyl ester
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.39 0.014 0.018 L-arginine
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+ absolutely dependent on Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.39 160000
-
 2 * 160000, SDS-PAGE Bos taurus
1.14.13.39 320000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.39 0.031
-
 purified pancreatic enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Bos taurus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.39 dimer 2 * 160000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 37
-
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.39 7.4
-
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 5,6,7,8-tetrahydro-L-biopterin not required for activity Bos taurus
1.14.13.39 Calmodulin required Bos taurus
1.14.13.39 FAD required Bos taurus
1.14.13.39 FMN required Bos taurus
1.14.13.39 NADPH
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.13.39 0.0015
-
 Ngamma-nitro-L-arginine
-
 Bos taurus
1.14.13.39 0.0034
-
 Ngamma,Ngamma-dimethyl-L-arginine
-
 Bos taurus
1.14.13.39 0.0054
-
 Ngamma-monomethyl-L-arginine
-
 Bos taurus
1.14.13.39 0.0155
-
 Ngamma-nitro-L-arginine methyl ester
-
 Bos taurus